RPLC-MS (Lipidomics)

RPLC-MS for Lipidomics

Column parameter

Phenomenex, UHPLC Kinetex C18 column (1.7 μm, 2.1× 100 mm)

or Waters, UPLC HSS T3 column (1.8 μm, 2.1× 100 mm)

Mobile phase

Positive MS mode and Negative MS mode

A:ACN: H2O = 6:4; 10mM HCOONH4

B:ACN: IPA = 1:9; 10mM HCOONH4

Gradient parameter

Time Flow rate (uL/min) A % B %
0.00 400 60.0 40.0
9.00 400 0.00 100.0
10.00 400 0.00 100.0
10.20 400 60.0 40.0
13.00 400 60.0 40.0