HILIC-MS (Zic-HILIC)

HILIC-MS (ZIC-HILIC column)


Column parameter

Merck, SeQuant ZIC-HILIC column (3.5 μm, 2.1× 100 mm)


Mobile phase

Positive mode and Negative mode

A:95% H2O, 5% ACN, 10 mM CH3COONH4 + 10 mM NH4OH

B:95% ACN, 5% H2O, 10 mM CH3COONH4 + 10 mM NH4OH


Gradient parameter

Time

Flow rate(uL/min)

A %

B %

0.00

300

1.00

99.0

13.0

300

60.0

40.0

15.0

300

60.0

40.0

15.1

300

1.00

99.0

20.0

300

1.00

99.0