HILIC-MS (Lunar amino column)

HILIC-MS (Lunar amino column)

Column parameter

Phenomenex, HPLC Luna aminopropyl column (3.0 μm, 2.0× 100 mm)

Mobile phase

Positive mode and Negative mode

A:100% H2O 25 mM CH3COONH4 + 25 mM NH4OH

B:100% ACN

Gradient parameter

Time Flow rate (uL/min) A % B %
0.00 300 15.0 85.0
2.00 300 15.0 75.0
9.00 300 100 0.0
14.0 300 100 0.0
15.0 300 15.0 85.0
20.0 300 15.0 85.0