HILIC-MS (Amide Column)

HILIC-MS (Amide Column)

Column parameter

Waters, UPLC BEH Amide column (1.7 μm, 2.1× 100 mm)

Mobile phase

Positive mode and Negative mode

A:100% H2O 25 mM CH3COONH4 + 25 mM NH4OH

B:100% ACN

Gradient parameter

Time Flow rate (uL/min) A % B %
0.00 300 15.0 85.0
1.00 300 15.0 85.0
12.00 300 35.0 65.0
12.1 300 60.0 40.0
15.0 300 60.0 40.0
15.1 300 15.0 85.0
20.0 300 15.0 85.0